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MSP-HTPrimer: a high-throughput primer style Device to enhance assay design and style for DNA methylation Assessment in epigenetics
g. the introduction of polyT?�s or polyA?�s stretches within the template immediately after bisulfite conversion). Our past work on the invention and validation of genome-extensive methylated biomarkers6 discovered various essential facets crucial for bisulfite PCR primer style, and based on these observations a set of seven criteria ended up outlined which a bioinformatics Resolution for high-throughput primer structure need to possess. These ended up:
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As a way to ameliorate the effectiveness of PCR, we designed a completely new primer-design program called BiSearch, accessible around the World Wide Web. It has the unique property of examining the primer pairs for mispriming web-sites to the bisulfite-dealt with genome and decides prospective non-particular amplification merchandise by using a new lookup algorithm. The options of primer-structure and Investigation for mispriming web-sites may be used sequentially or sep...
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